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. 2021 Oct 20;9:747969. doi: 10.3389/fcell.2021.747969

FIGURE 5.

FIGURE 5

Network-wide response to transient RA manipulations. (A) RA metabolic network components and RA target genes analyzed during early/mid gastrula. The homeolog (L&S) or singleton status of the gene(s) in the genome is denoted outside the box with the gene name. The gene names shown include a .L suffix to mark in cases where only one of the homeologs changed significantly in the RNAseq and HT-qPCR data. (B) Analysis of the expression change during the recovery period following the transient increase (RA) and decrease (DEAB) in RA levels. The hox gene and the RA metabolic network component changes were averaged from the RNAseq and HT-qPCR analyses. The black frame around the t = 0 h time point denotes the network-wide response at the end of the treatment. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; not labeled, not significant. (C,D) Mapping the differential expression data onto the RA network shown in panel (A) to highlight the differences in regulation of biosynthesis versus degradation of RA signal between RA and DEAB treatment groups.