FIGURE 8.

Differential robustness to direction of RA change is related to the effectiveness of feedback regulatory action. (A,B) Comparative distribution of clutches based on the trajectory determined robustness to RA (A) and DEAB (B). The change of the hox genes as a function of the change in the RA network genes is plotted for each RA manipulation. The letters indicate the distinct clutches. (C) Schematic diagram of the robustness efficiency-efficacy matrix in which different quadrants indicate the relative level of feedback observed and robustness achieved. (D,E) Mapping the differential expression data of clutches A, E, and F onto the RA network shown in Figure 5A to highlight the heterogeneity of differential regulation of RA network components for clutches closely situated in the robustness efficiency matrix (A,B).