Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 20;15:732183. doi: 10.3389/fncir.2021.732183

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Li, Walker, Zhao, Edwards, Michki, Cheng, Ghazzi, Chen, Chen, Roossien and Cai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Super-resolution Bitbow imaging enabled by a modified protein retention-Expansion Microscopy (pro-ExM) protocol. (A) Experimental flow of the modified pro-ExM protocol. (B) Serotonergic neurons labeled by TRH-Gal4 driven 2x Bitbow2.1 without sample expansion and imaged by native fluorescence. (C) Serotonergic neurons labeled by TRH-Gal4 driven 2x Bitbow2.1 after ∼4x sample expansion and imaged by immuno-fluorescence. (D,E) Magnified boxed regions in panles (B,C), respectively. Arrowheads indicate VNC serotonergic neurons within the same hemi-segment send out co-fasciculated neurites that form a single commissure projecting to the contralateral side. Scale bars, (B,C) 100 μm, (D,E) 10 μm.