FIGURE 5.

Bitbow2 tracing enables serotonergic neuron morphology and network analysis in larval VNC. (A) 3D rendering of traced VNC serotonergic neurons labeled by Bitbow2. Top right, dashed-line box indicates the VNC volume that has been traced. (B) Cross-section view of the traced serotonergic neurons located in the A5 segment (solid outline), which illustrates that the somas (solid oval shapes) are located at the very ventral part of the VNC while the neurites occupy mostly in the sensory zone (ventral part) of the neuropil (dashed outline). (C) Z-projections (dorsal view) of 21 traced serotonergic neurons categorized into 8 morphological subtypes that are indicated by distinct pseudo-colors. (D) Schematic of the abdominal VNC and serotonergic neurons. Circles indicate the soma locations of the traced serotonergic neurons and their colors correspond to the morphological subtype pseudo-colors in panel (C). Dashed circles indicate the soma locations of the unlabeled serotonergic neurons.