Table 3.

Essential genes encoding products predicted to be released from the cell or expressed at the cell surface.

Gene ID†	Pan-genome	Shared	Annotation	Cell location
RS10405	Core	1	rsxB—Electron transport complex subunit	Cell inner membrane
RS16490	Core	0	cdsA—Phosphatidate cytidylyltransferase	Cell inner membrane
RS17415	Core	2*	rseC—SoxR reducing system protein	Cell inner membrane
RS00530	Core	1*	pspE—Thiosulfate sulfurtransferase	Periplasm
RS02940	Unique	0	Putative excinuclease	Cell inner membrane
RS08840	Accessory	1*	bcrA—benzoyl-CoA reductase subunit A	Cell inner membrane
RS17130	Core	4	murG ‡	Cell inner membrane

Seven essential genes of V. anguillarum NB10Sm contained a significant peptide-signal and secretory pathway signal (P-value <10^-3), with predicted subcellular locations as reported for E. coli. The seven genes were classified according to the pan-genome (see Figure 4) and were investigated for conservation within the six Vibrio essential gene studies (“Shared” column; see Figure 5), including three genes found to be shared with the V. anguillarum essential gene study of Guanhua et al. (2018) (*).

^†GeneID: VANGNB10_RSxxxxx.

^‡UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase.