FIGURE 3.

Specific expression of AG genes revealed by semi-quantitative RT-PCR and fig fruit transcriptome. (A) AG genes are widely expressed in fig flowers, but male-specific AG genes FcAG2/3-Gall_Stamen are only found in fig galls and stamens. (B) Transcriptome data of “Purple Peel” fruit reveal that the FcAG gene is only expressed in the pistils; no expression was detected in the peel. (C) AG-Marker PCR products are only identified in male individuals; both DNA and cDNA can be used as the template. (D) Sectional view of a female fig fruit (left) and male fig fruit (right). Both were confirmed by our sex-identification markers.