TABLE 1.

Primers used in this study.

Primer name	Primer sequence (5′→3′)	Length (bp)	Target length (bp)
AG-F	ATGKCGTWCCAAAACAAGGWGAWGA	25	759
AG-R	TTASACTAATTGGAGGGCCATGGRA	25
AG-Marker-F	CAGGAGGAGGAAGCT	15	146
AG-Marker-R	TTACACTAATTGGAGGGCCA	20
FcRAN1-F	ATGGCGGCGAGCGTCCGACA	20	3015
FcRAN1-R	CTATTCTACAGTTATTTCTAGTATA	25
RAN1-Marker-F	ATATCAAGAATGCAATCGAGGA	22	608
RAN1-Marker-R	GCTGAGAAATAGACTAGAGATG	22
qPCR-F	CCACTGGCAAAGGCAATAGT	20	156
qPCR-R	TTGAACTCCTCTGCCGGGAA	20
1-SNP-F	TCCTGTCTTTCTCATACGAGTAGTT	25	371
1-SNP-R	TGGCGTCAGATGTTTTCCCC	20
6SNP-2-F	CTGGAGCTCTTCGTGCTTAC	20	766
6SNP-2-R	AAGCATTGAACTCCTCTGCCG	21
2-SNP-F	TCACGATGTAAGGGCAGAGG	20	444
2-SNP-R	GTGGTCAGCCTTGGTTTTCTG	21
Actin-F	GAACCACCAGACAGGACGATG	21	250
Actin-R	CTACCACTGCTGAACGGGAAA	21

AG primers contain degenerate bases: K(G/T), W(A/T), S(G/C), R(A/G).