FIGURE 1.

Validation of the the tomato spotted wilt virus (TSWV) clustered regularly interspaced short palindromic repeats (CRISPR)/clustered regularly interspaced short palindromic repeat-associated 13a (Cas13a)-based detection system. The positive sample in a 100-μl detection buffer contained a plasmid of the TSWV N gene as the template for RPA reaction, T7 RNA polymerase, NTP, recombinant RNase inhibitor, purified LwCas13a, crRNA, and RNA reporter. The detection systems were monitored in the absence of LwCas13a, crRNA, RNA probe, or RPA amplification products. The negative control utilized RNase-free water as a template of the RPA reaction. RPA, recombinant polymerase amplification.