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. 2021 Oct 20;12:745173. doi: 10.3389/fmicb.2021.745173

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Copyright © 2021 Zhang, Jiao, Ding, Shen, Li, Yu, Huang, Li, Wang and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Length of detection of PCR and RPA to detect different concentrations. (A) Different concentrations amplified by RPA. (M1) DL2000 Plus DNA Marker. (1–8) The plasmid with N segment of the TSWV were serially diluted 10-fold (2.26 × 10⁸ to 2.26 × 10¹ copies/μl). (9) Negative control has RNase-free water as an input. (B) Different concentrations only amplified by the PCR reaction. (M2) DL2000 Plus DNA Marker. (1–8) The plasmid with N segment of the TSWV were serially diluted 10-fold (2.26 × 10⁸ to 2.26 × 10¹ copies/μl). (9) Negative control has RNase-free water as an input. RPA, recombinant polymerase amplification.