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. 2021 Oct 20;12:745173. doi: 10.3389/fmicb.2021.745173

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Copyright © 2021 Zhang, Jiao, Ding, Shen, Li, Yu, Huang, Li, Wang and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The sensitivity of CRISPR/Cas13a-based detection. (A) Fluorescence intensity for plasmid templates of RPA reactions with different concentrations. (B) Intensity of fluorescence signals generated after 15 min (30 kinetics cycles) of CRISPR/Cas13a-based detection at each dilution. The negative control has RNase-free water as an input instead of RPA production. Data are the mean ± SD from triplicate biological replicates measurements. Comparisons between the groups were made using an analysis of variance (ANOVA) followed by a Tukey’s test. Different letters above the error bars indicate significant differences at the 0.05 level. RPA, recombinant polymerase amplification; SD, standard deviation.