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. 2021 Oct 20;12:745173. doi: 10.3389/fmicb.2021.745173

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Copyright © 2021 Zhang, Jiao, Ding, Shen, Li, Yu, Huang, Li, Wang and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The specificity of CRISPR/Cas13a-based detection for the TSWV. (A) Fluorescence intensity for different viruses. (B) Intensity of fluorescence signals generated after 15 min (30 kinetics cycles) of CRISPR/Cas13a-based detection for different viruses. Negative control has RNase-free water as input instead of RPA production. Data are the mean ± SD from triplicate biological replicates measurements. Comparisons between groups were performed using an analysis of variance (ANOVA) followed by a Tukey’s test. Different letters above the error bars indicate significant differences at the 0.05 level. RPA, recombinant polymerase amplification; SD, standard deviation; TSWV, tomato spotted wilt virus.