FIGURE 5.

Detection of samples using CRISPR/Cas13a-based detection in plants and Frankliniella occidentalis. (A) Intensity of fluorescence signals generated after 15 min (30 kinetics cycles) of the CRISPR/Cas13a-based detection of 10 tomato samples. The negative control has healthy tomato as input. (B) Intensity of fluorescence signals generated after 15 min (30 kinetics cycles) of CRISPR/Cas13a-based detection of 12 F. occidentalis samples. Data are the mean ± SD. from triplicate biological replicates measurements. Negative control has healthy F. occidentalis as an input. Comparisons between groups were made using an analysis of variance (ANOVA) followed by a Tukey’s test. Different letters above the error bars indicate significant differences at the 0.05 level.