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. 2021 Oct 20;12:748393. doi: 10.3389/fpls.2021.748393

FIGURE 1.

FIGURE 1

Domain-architecture and structure prediction of CdgC. (A) Genetic context of the AZOBR_100206 (marked in blue) gene which codifies for the CdgC protein. Downstream of it and separated by an intergenic region of 68 pb, AZOBR_100205 gene is found. (B) Scheme representation of the genetic context after the deletion of 509 pb belonging to the ORF of cdgC gene. This deletion leaded to the loss of the nucleotides that encode for the catalytic and inhibitory sites of the GGDEF, and the aspartate 51 located at the REC domain. Bar represents 500 pb. (C) Graphic representation of the domain architecture of CdgC obtained from the webserver SMART. (D) Superimposed ribbon diagrams from the REC domain of CdgC (pink) and the REC domain of WspR (blue) from P. aeruginosa. The residue involved in phosphorylation is marked in green. (E) Superimposed ribbon diagrams from the DGC domain of CdgC (pink) and the DGC domain of WspR (blue) from P. aeruginosa. Active and inhibitory sites are colored in yellow. Modeling of the REC and DGC domains of CdgC was carried out employing WspR as a model. REC domain (C-score: 1.38; TM-score: 0.91 ± 0.06; RMSD: 1.7 ± 1.4 Å), DGC domain (C-score: 1.71; TM-score: 0.95 ± 0.05; RMSD: 1.8 ± 1.5 Å). (F) Web Logo showing conserved inhibitory and active sites of CdgC compared to WspR.