FIGURE 2.

Biofilm formation analysis with confocal microscopy. Maximum projection confocal laser scanning microscopy images of 5-day old static biofilms grown in NFB media and tagged with an eGFP fluorescent protein (shown in green) and dyed with calcofluor CWC (shown in blue). The A. baldaniorum WT, A. baldaniorum 59C mutant, A. baldaniorum C21 mutant complemented with cdgC gene, A. baldaniorum C87 mutant without cdgC gene strains. Scale bar 10 μm. Intensity surface plot corresponding to the emitted fluorescence by CWC is shown in the last panels. The Nikon Eclipse Ti-E C2+ (CLSM) was used with a 60x Plan lambda objective. These are representative images from at least three independent experiments.