FIGURE 3.

Biofilm formation and exopolysaccharide production assays. (A) Bar plots representing the mean and standard deviation of biofilm quantification assays of Azospirillum strains (A. baldaniorum WT, A. baldaniorum 59C mutant, A. baldaniorum C21 mutant complemented with cdgC gene, A. baldaniorum C87 mutant without cdgC gene) grown in NFB* media supplemented with KNO₃ or NFB media. (B) Bar plots representing the mean and standard deviation of calcofluor-binding assays from same above Azospirillum strains grown in NFB* media supplemented with KNO₃ or NFB media. The data shown were obtained from three independent experiments. The means of the wild-type strain and the mutant strain were compared using a Student’s t-test. Asterisks indicate a P-value = 0.00042 in (A) and P-value = 0.00868 in (B).