FIGURE 6.

Confocal images of colonization of wheat roots by CLSM. (A) Colonization pattern of the mCherry-tagged A. baldaniorum 2449 in wheat roots after 7 days of inoculation. Typical endophytic behavior of the wild-type strain. (B) Wild-type strain (red fluorescence) location was corroborated through a longitudinal cut of wheat root. White arrowheads indicate mCherry-expressing A. baldaniorum 2449 strain. The images (A,B) represent the merge of two micrographs of mCherry detection (shown in red) and plant autofluorescence (shown in green). (C) Colonization pattern of the eGFP-tagged A. baldaniorum 2450 in wheat roots after 7 days of inoculation, showing an alteration in its ability to internalize, locating outside of wheat root. (D) Surface colonization pattern of A. baldaniorum 2450 expressing eGFP in wheat roots after 7 days of inoculation, indicated with arrowheads. Magnification 10X. The images (C,D) represent the merge of two micrographs of eGFP detection (shown in green) and plant autofluorescence (shown in red). Bars represents 50 μm. Representative images were acquired by examination of several images with a 20X objective, and 1.8 amplification zoom.