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. 2021 Oct 20;12:721426. doi: 10.3389/fmicb.2021.721426

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Copyright © 2021 Cai, Zheng, Lu, Zhao, Sun, Tang, Xiao, Wang, Tong, Zhao, Xiao, Zhao and Xue.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Construction and oxacillin susceptibilities of mecA1 overexpression strains. The mecA1 gene from S. sciuri NWAF26 was introduced into the methicillin sensitive S. aureus strains RN4220 and Newman by shuttle vector pSE1, respectively. (A) The expression level of mecA1 were measured by qRT-PCR. (B) Oxacillin susceptibilities were determined using MH agar with 2% NaCl containing 1 mg/L oxacillin. NWAF26^R was taken as a positive control.