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. 2021 Oct 20;12:760017. doi: 10.3389/fmicb.2021.760017

FIGURE 2.

FIGURE 2

In TMP + ERY combination treatment, TMP exerts a much stronger overall effect on the transcriptomic response than ERY does. (A) TMP triggers a different transcriptomic response than ERY but is similar to the combination treatment of TMP + ERY. Principal component 1 (PC1) explains 78% of the total variance in gene expression, while PC2 accounts for 16%. TMP (blue) and ERY (yellow) trigger different transcriptomic responses, and the response to TMP + ERY treatment (green) is more similar to TMP than it is to ERY. (B) Except for three sulfur metabolism related genes, TMP and TMP + ERY show a high degree of similarity in gene expression profiles. Normalized gene counts from RNA-Seq were Z-score transformed across all sample groups before a two-way hierarchical clustering analysis was performed based on the top 100 genes with the highest variance. The heatmap revealed that the transcriptomic responses for the TMP and TMP + ERY groups were highly similar across four MultiFun gene ontology (GO) categories for 97 genes. The only exception is the sulfur metabolism category in which cysH and the cysIJ operon do not display TMP-induced upregulation in the TMP + ERY group (inset).