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. 2021 Oct 20;12:760017. doi: 10.3389/fmicb.2021.760017

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Copyright © 2021 Qi, Angermayr and Bollenbach.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Perturbing the sulfate reduction pathway by adding exogenous sulfate increases cysH expression level in TMP + ERY treated MG1655 and changes the TMP + ERY interaction from additive to suppressive. (A) qRT-PCR validation of cysH expression. Relative to the TMP-treated MG1655 reference group, the TMP + ERY treated group showed an approximately twofold reduction in cysH expression, which confirmed the role of ERY in suppressing cysH expression. Supplementing the LB growth medium in which the TMP + ERY treated group is grown with 2 mM sulfate caused an increase in average cysH relative expression. (B) ERY exerts suppression on TMP in growth medium supplemented with sulfate. Isoboles for TMP + ERY drug combinations when tested against the E. coli MG1655 strain. Supplementing LB medium with sodium sulfate at 2 mM changed the interaction between TMP and ERY from additive to suppressive. Suppression by ERY on TMP is stronger at higher concentrations of TMP in the presence of exogenous sulfate.