FIGURE 2.

Network reconstruction. (A) An example of multi-colored labeled neuron culture and reconstruction of a neuronal network. Edge (L): 55 μm. Gap (D): 30 μm. (B) A representative network of a culture neuronal circuit. Color of a node and size of a node indicate in-degree and out-degree, respectively. (C) Scatter plots and histograms of a number of patterns occupied by dendrite of individual neurons (RPA) and their in-degree values. Black line (linear fit); y = 0.4363×x+0.8179, R² = 0.09573. (D) Scatter plots and histograms of a number of patterns covered by axons from individual neurons (axon coverage) and out-degree values. Black line (linear fit) y = 0.1804×x+0.0.9031, R² = 0.2178. (E) Scatter plots of numbers of neighboring neurons in first nearest neighboring hexagonal patterns and in-degree values. (F) Scatter plots of numbers of neighboring neurons in first and second nearest neighboring patterns and out-degree values. The data points were jittered in the 0.5 range to avoid overlap. (N: 434 cells from 6 culture samples).