Table 3.

Conditioning each of the traits associated with E354 at nominal significance from the 2SMR analysis on independent SNPs for each trait

	2SMR result		Conditional result		Independent variant
Trait	β (SE)	P	β (SE)	P ‡	Conditioned on*	LD with rs1800437†
T2D	−0.03 (0.007)	7 × 10−5	−0.03 (0.008)	4 × 10−4	rs3810291	0.001
T2DadjBMI	−0.07 (0.009)	2 × 10−14	−0.02 (0.009)	0.04	rs2238689	0.363
CAD	0.03 (0.007)	2 × 10−6	0.01 (0.007)	0.06	rs1964272	0.269
SVS	−0.08 (0.029)	0.009	−0.04 (0.029)	0.12	rs1964272	0.269
Nonfasted plasma glucose	0.02 (0.003)	3 × 10−8	0.01 (0.003)	0.05	rs1964272	0.269
HbA1c	−0.01 (0.003)	1 × 10−7	−0.0003 (0.003)	0.92	rs9676912	0.356
ApoA1	0.01 (0.003)	3 × 10−6	0.002 (0.003)	0.37	rs2238689	0.363
HDL	0.01 (0.003)	5 × 10−7	0.003 (0.003)	0.25	rs2238689	0.363
ApoB	0.02 (0.002)	5 × 10−13	0.01 (0.002)	2 × 10−5	rs7412	0.004
LDL	0.02 (0.003)	2 × 10−16	0.016 (0.003)	1 × 10−8	rs7412	0.004
Triglycerides	−0.01 (0.003)	2 × 10−5	−0.01 (0.003)	5 × 10−5	rs4803936	0.001
CRP	−0.01 (0.002)	0.02	−0.004 (0.002)	0.07	rs7412	0.004
Albumin	−0.01 (0.003)	6 × 10−6	−0.01 (0.003)	0.001	rs35114617	0.061
Creatinine	−0.02 (0.002)	1 × 10−11	−0.02 (0.002)	3 × 10−11	rs7412	0.004
QPCTL	−0.07 (0.016)	9 × 10−6	0.01 (0.016)	0.48	rs1964272	0.269
Secretoglobin family 3A member 1	−0.08 (0.017)	6 × 10−7	−0.04 (0.017)	0.01	rs61703905	0.1

Estimates of 2-h glucose, total cholesterol, and BMI were not included in this table, as the independent signal selection showed that E354 was one of the independent variants. adj, adjusted for; CRP, C-reactive protein; QPCTL, glutaminyl-peptide cyclotransferase like; SVS, small vessel stroke.

^*The independent variant showing the greatest attenuation of the E354 association estimate with the respective trait.

^†LD estimates are in R² and are quoted from five European populations in the LDlink database v4.1.0.

^‡A nominal significance threshold of P ≤ 0.05 was used to ascertain significance for the conditional results.