Figure 5.

INS⁺ENTPD3⁺ cells show improved function. A: Schematic representation of pINS⁺ENTPD3^+/− cells sorted from seBC clusters reaggregated (reagg) in the presence of support cells, HUVEC, and mesenchymal stem cells for 48 h. B: Perifusion analysis of INS⁺ENTPD3⁻ and INS⁺ENTPD3⁺ clusters; 20–25 clusters were analyzed per condition, and data are presented as % of total insulin in cluster pellet recovered (n = 2 independent differentiation experiments). C: Total insulin content of clusters recovered following perifusion analysis (n = 2 independent differentiation experiments). D: Relative insulin secretion during perifusion of INS⁺ENTPD3⁻ and INS⁺ENTPD3⁺ clusters (n = 2 independent experiments) (data normalized to basal [0.5 mmol/L glucose] secretion). Gluc, glucose; hPSC, human pluripotent stem cell.