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. 2021 Aug 25;70(11):2595–2607. doi: 10.2337/db21-0261

Figure 1.

Figure 1

PLIN2 influences LD accumulation in human EndoCβH2-Cre β-cells. A: Experimental workflow and depiction of how maturation changes upon Cre-mediated SV40 Tag removal. B: The expression level of all members of the PLIN family before and after Cre treatment. Both PLIN2 and PLIN3 levels increased significantly. C: The change in PLIN mRNA levels upon PLIN2KD and PLIN2OE. D: Quantitation of the change in LD levels between Sham and PLIN2KD or PLIN2OE cells incubated without (control [CT]) or with 500 μmol/L EA or PA for 24 h. E: Representative images of D. Notably, FFA-induced LD accumulation appears to positively correlate with PLIN2 levels, which was statistically significant in the EA-treated group. All error bars indicate SD. n = 3–4. *P < 0.05, **P < 0.01, ***P < 0.05 vs. βH2 or βH2-Cre PLIN1 (B), Sham (C), or control (D) unless specified. βH2, EndoCβH2; βH2-Cre, EndoCβH2-Cre; Lv, lentivirus; qPCR, quantitative PCR.