Figure 5.

Cellular distribution of proteins linked to β-cell identity in PLIN2KD cells. Immunofluorescent staining illustrates that somatostatin (SST) (red) and glucagon (GCG) (white) (A) and FEV (red) (B) protein production is only found in a small fraction of PLIN2KD cells, with quantitation of the change in SST⁺, GCG⁺, or FEV⁺ cell percentages between Sham and PLIN2KD or PLIN2OE cells (C); all error bars indicate SD. n = 3. *P < 0.05, **P < 0.01 vs. Sham. D: In contrast, MAFA (red, top panel) and NKX2.2 (red) protein levels were lower in most PLIN2KD cells. The remaining MAFA⁺ and NKX2.2⁺ cells in the PLIN2KD population probably represent uninfected cells. DAPI (blue) and BODIPY (green) counterstaining. Scale bar = 50 µm.