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. 2021 Aug 25;70(11):2595–2607. doi: 10.2337/db21-0261

Figure 6.

Figure 6

TUDCA rescues PLIN2KD-induced dysfunction, while PLIN2OE prevents EA toxicity. Sham, PLIN2KD, and PLIN2OE cells were treated with TUDCA (100 μmol/L) and/or EA (500 μmol/L) for 24 h. The toxic effects of PLIN2KD and EA on GSIS (A) and insulin levels (B) were rescued by TUDCA and PLIN2OE treatment, respectively. C: Expression of the majority of candidate regulatory genes was corrected to Sham by TUDCA or PLIN2OE. Ins, insulin. D: On the left is a representative immunoblot and on the right the quantification of multiple sXBP1 and ACTIN analyses performed on Sham, PLIN2KD, and PLIN2OE cells. All error bars indicate SD. n = 3–4. *P < 0.05, **P < 0.01, ***P < 0.001 vs. corresponding LG (A) or Sham (B–D) unless specified. ET, erucic acid + TUDCA; HG, high glucose; LG, low glucose; n.s., nonsignificant.