Figure 3.

RNA profiling of pancreatic β-cells in the presence (wild-type) and absence (KO) of Cavβ3. A: Expression levels of pancreatic α-, β-, δ-, and γ-cell-specific genes in wild-type and Cavβ3-KO (n = 6) Ins-1 cells. Data are shown as single values of FPKM and bar graphs with mean ± SD. B: Differentially expressed genes identified by RNA sequencing in wild-type (WT; n = 3) and Cavβ3-KO (KO; n = 3) β-cells with an FDR-adjusted P value <0.05. C: Expression levels of Ins1, Ins2, Mafa, Camk4, Pcsk1, Nos1, Txnip, and Vamp8 genes from WT (black; n = 3) and Cavβ3-KO (red; n = 3) cells, shown as single FPKM values and bar graphs with mean ± SD, with the indicated P values calculated by unpaired two-tailed Student t test. D: Gene expression levels of high voltage-gated Ca²⁺ channel subunits and of intracellular ion channels obtained by RNA sequencing from WT (black; n = 3) and Cavβ3-KO (red; n = 3) cells, shown as single FPKM values and bar graphs with mean ± SD.