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. 2021 Aug 11;70(11):2476–2491. doi: 10.2337/db20-1228

Figure 5.

Figure 5

Hepatocellular eNOS overexpression increases primary hepatocyte respiration. Primary hepatocytes were isolated from female eNOSfl/fl mice (20–22 weeks of age) and transfected with either Adv β-Gal control or Adv eNOS overexpression. A: Representative Western blot image of Adv eNOS overexpression in primary hepatocytes. eNOS expression in cells exposed to Adv-β-Gal are underexposed due to the robust increases in eNOS expression in cells exposed to Adv-eNOS. B: Oxygen consumption rate (OCR) in isolated primary hepatocytes (n = 3). C: Gene expression and protein expression in isolated primary hepatocytes (n = 3). CD-fed 10-week-old male C57BL/6J mice were injected with either AAV8-TTR-eNOS or AAV8-TTR-GFP control. At 6 weeks post–AAV8-TTR-eNOS overexpression. D: eNOS gene expression in whole liver and isolated primary hepatocytes, and (E) oxygen consumption rate in isolated primary hepatocytes (n = 7–8/group). Data are presented as mean ± SD. *Significantly different from β-Gal and from AAV8-GFP; #Significantly different from β-Gal, 104, 105; $Significantly different from 105 (for all P < 0.05). AAV, adeno associated virus; Adv, adenoviral; AU, arbitrary units/correction; CD, control diet; FCCP, carbonyl cyanide-p-trifluoromethoxyphenylhy drazone; OE, overexpression; Rot/Ant, rotenone/antimycin; RQ/PPIB, relative quotient/cyclophilin B.