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. 2021 Aug 11;70(11):2476–2491. doi: 10.2337/db20-1228

Figure 8.

Figure 8

Hepatic eNOS is reduced in human patients with elevated NAFLD activity scores and associated with hepatic mitochondrial dysfunction and hepatic BNIP3. Liver samples were obtained from adults undergoing elective bariatric surgery, with participants clustered into 3 groups based on histological NAS; no disease (NAS = 0, n = 7), moderate (NAS = 1–3, n = 45), and severe (NAS = 4-6, n = 38). A: Representative liver H&E and trichrome staining for each of the 3 NAS groupings (scale bar represents 50 μm). B: Whole-liver total and phosphorylated (ser1117) eNOS protein content ran on a continuous gel across each NAS cluster (n = 7, 45, 38, respectively). Pearson's correlation of eNOS protein content with (C) isolated hepatic mitochondrial fatty acid oxidation and (D) BNIP3 protein (n = 90). Data are presented as mean ± SD. *Significantly different from NAS 0 (P < 0.05). AU/correc., arbitrary units/correction; ASMs, acid-soluble metabolites; H&E, hemotoxylin and eosin; NAS, NAFLD activity score.