Skip to main content
. 2021 Aug 30;70(11):2580–2594. doi: 10.2337/db21-0422

Figure 2.

Figure 2

Heterozygous (Het) Ins2-proinsulin-R(B22)E mice fed normal chow (NC) or HFD. A: Weekly body weight measurements in male mice (n = 4–8 per group). B: Intraperitoneal glucose tolerance test in 5.5-week-old males on NC (n = 4–5 per group). C: Area under the curve (AUC) from the data in B. D: Weekly random blood glucose in males on NC or HFD (n = 4–8 per group), with Ins2-proinsulin-R(B22)E-Het on NC shown in blue and on HFD in red. E: Intraperitoneal glucose tolerance test in 11-week-old males on NC or HFD for 6 weeks (n = 3–5 per group), with Ins2-proinsulin-R(B22)E-Het mice on NC in blue and on HFD in red. F: AUC from the data in E. G: Random serum insulin level in 11-week-old WT (purple) or Ins2-proinsulin-R(B22)E-Het (red) males on HFD for 6 weeks (n = 5–7 per group). H: The data from G used to calculate serum insulin-to-glucose ratio. I: Insulin secretion from isolated islets of WT (purple) or Ins2-proinsulin-R(B22)E-Het (red) males on HFD at 2.8 mmol/L (unstimulated) and 16.7 mmol/L (stimulated) glucose (n = 3–4 per group). J: Isolated islets from male WT and Ins2-proinsulin-R(B22)E-Het mice fed NC or HFD were lysed and analyzed by reducing SDS-PAGE and immunoblotting with monoclonal antibody anti-proinsulin (top panel), guinea pig anti-insulin (middle panel), and anti-cyclophilin B (CypB) (loading control) (bottom panel). K: Weekly body weight measurements in female Ins2-proinsulin-R(B22)E-Het mice fed NC (blue) or HFD (green) (n = 4 per group). L: Weekly random blood glucose measurements in female Ins2-proinsulin-R(B22)E-Het mice on NC (blue) or on HFD (green) (n = 4 per group). Data are mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA.