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. 2021 Aug 30;70(11):2580–2594. doi: 10.2337/db21-0422

Figure 3.

Figure 3

Homozygous (Hom) Ins2-proinsulin-R(B22)E mice develop spontaneous diabetes. A: Random blood glucose at 5 weeks of age in WT (black), heterozygous (Het) (blue), and Hom (red) Ins2-proinsulin-R(B22)E mice fed normal chow (n = 3–7 per group). Males and females shown separately as well as combined. B: The same mice from A remeasured 8 weeks postpartum. C: Serum insulin level measured at 5.5 weeks of age in WT (black), Het (blue), or Hom (red) males fed normal chow (n = 5 per group). Males and females shown separately as well as combined. D: Insulin-to-glucose ratio calculated from the samples in B and C, respectively. E: GSIS in vivo at 0 and 15 min poststimulation from WT (black), Het (blue), or Hom (red) Ins2-proinsulin-R(B22)E mice fed normal chow (6-week-old males, n = 5–6 per group). F: GSIS from islets isolated from WT (black), Het (blue), or Hom (red) Ins2-proinsulin-R(B22)E mice fed normal chow at 2.8 mmol/L (unstimulated) and 16.7 mmol/L (stimulated) glucose concentration (6-week-old males, n = 5–7 per group). G: Serum proinsulin level from the same animals measured in C (males and females combined). H: Proinsulin-to-insulin ratio from the animals in G (males and females combined). Data are mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA.