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. 2021 Aug 30;70(11):2580–2594. doi: 10.2337/db21-0422

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© 2021 by the American Diabetes Association

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Progressive loss of insulin, accompanied by proinsulin misfolding, in Ins2-proinsulin-R(B22)E homozygotes (Hom). A: Reducing SDS-PAGE and immunoblotting with anti-proinsulin (top panel), anti-insulin (middle panel), and anti-cyclophilin B (CypB) (loading control, bottom panel). Islets were isolated from males (ages 5–7 weeks) with the genotypes shown above; random blood glucose level at the time of euthanasia is also indicated. B: Nonreducing SDS-PAGE and immunoblotting of the identical samples as that shown in A, highlighting aberrant proinsulin disulfide-linked complexes. C: Immunoblotting of p58ipk and BiP from male WT and Ins2-proinsulin-R(B22)E heterozygotes (Het) and Hom (age 5–6 weeks). D: Quantitation of immunoblotting like that shown in C (n = 6 per group). Statistical analysis (mean ± SD) shows that changes in normalized BiP protein levels were nonsignificant. **P < 0.01. Proins, proinsulin.