Table 1.
Key resources
| Reagent type (species) or resource | Designation | Source or reference | Identifier | Additional information |
|---|---|---|---|---|
| Cell line (mouse) | Min6 (mouse β-cell line) | Soleimanpour et al.56 | ||
| Antibody | Anti-rat proinsulin monoclonal CCI-17 | ALPCO | CCI-17 | 1:1,000 |
| Antibody | Anti-human proinsulin | Abmart (Haataja et al.34) | 1B24, 3L10 | 1:1,000 |
| Antibody | Guinea pig anti-insulin | Covance | 1:500 | |
| Antibody | Mouse antiglucagon | Abcam | ab109888 | 1:1,000 |
| Antibody | Antiglucagon (rabbit polyclonal) | Millipore | AB932 | 1:500 |
| Antibody | Anti-KDEL (rabbit monoclonal) | Novus Biologicals | NBP2–75549 | 1:300 |
| Antibody | Anti-Myc (rabbit polyclonal) | Immunology Consultants Laboratory | RMYC-45A | 1:500 |
| Antibody | Rabbit anti-cyclophilin B | Thermo Fisher Scientific | PA1-027A | 1:1,000 |
| Antibody | Alexa Fluor 488, 555, 647 (secondary) | Invitrogen | 1:500 | |
| Antibody | HRP guinea pig, rabbit, or mouse 2° | Jackson ImmunoResearch | 1:5,000 | |
| Recombinant DNA reagent | pTARGET (vector) | Promega | A1410 | |
| Recombinant DNA reagent | Myc-WT proinsulin (plasmid) | Liu et al.57 | Human proinsulin | |
| Commercial assay or kit | QuikChange II Site-Directed Mutagenesis Kit | Agilent Technologies | Agilent: 200524 | |
| Commercial assay or kit | Mouse insulin ELISA | ALPCO | 80-INSMS-E10 | |
| Commercial assay or kit | Enhanced chemiluminescence Immobilon or Clarity | Millipore or Bio-Rad, respectively | ||
| Chemical compound, drug | N-ethylmaleimide | Sigma-Aldrich | E3876 | |
| Chemical compound, drug | Trans 35S Label | Perkin-Elmer | PerkinElmer: NEG072007MC |
|
| Chemical compound, drug | ProSieve 50 Gel Solution | Lonza | Lonza: 50618 | |
| Chemical compound, drug | cOmplete Mini Protease Inhibitor Cocktail | Roche | Roche: 11836153001 |
|
| Chemical compound, drug | Collagenase P | Sigma-Aldrich | 11249002001 | |
| Chemical compound, drug | Protein A agarose | Invitrogen | ||
| Chemical compound, drug | Dithiothreitol, pansorbin, urea | Sigma-Aldrich or Thermo Fisher Scientific | ||
| Reagent | Lipofectamine 2000 | Thermo Fisher Scientific | ||
| Reagent | Tissue culture reagents | Thermo Fisher Scientific | ||
| Reagent | Met/Cys-deficient RPMI medium | Sigma-Aldrich | ||
| Reagent | ProLong Gold Antifade Reagent with DAPI | Thermo Fisher Scientific | Invitrogen P36931 | |
| Reagent | Unmasking solution | Thermo Fisher Scientific | Cell Signaling 14747P | |
| Cell culture imaging | Nunc LabTek-II Chambers | Thermo Fisher Scientific | ||
| Software, algorithm | GraphPad Prism 8.0 | GraphPad Software |