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. 2021 Aug 30;70(11):2626–2638. doi: 10.2337/db20-1235

Figure 4.

Figure 4

Generation of a βSENP1-KO. A: Expected tissue selectivity of SENP1 KO in the pSENP1-KO and βSENP1-KO mice. B: qPCR of Senp1 expression in tissues from pSENP1-WT and -KO mice (n = 3 and 3). C: Oral glucose–stimulated total plasma GLP-1 in CD (n = 10 and 9) and HFD (n = 10 and 9) mice. D: Oral glucose–stimulated plasma GIP in CD and HFD mice (n = 5–9 mice). E: Fasting plasma glucagon from pSENP1-WT and -KO mice fed CD or HFD (n = 13, 11, 12, and 9). F and G: Glucagon secretion at indicated glucose levels in the presence of GIP (n = 3 and 3) (F) or GIP + alanine from islets of pSENP1-WT and -KO mice on CD (n = 4 and 4) (G). H: qPCR of Senp1 expression in tissues from βSENP1-WT and -KO mice (n = 3 and 3). I: Fasting plasma glucagon from βSENP1-WT and -KO mice fed CD or HFD (n = 4, 5, 6, and 4). J and K: Glucagon secretion at indicated glucose level in the presence of GIP (n = 4 and 4) (J) or GIP + alanine from islets of βSENP1-WT and -KO mice on CD (n = 5 and 4) (K). Data from male and female islets were combined and are mean ± SEM and were compared using Student t test or one-way or two-way ANOVA followed by Bonferroni posttest. *P < 0.05, **P < 0.01, ***P < 0.001 between pSENP1-WT and pSENP1-KO or between βSENP1-WT and βSENP1-KO. Glu, glucose.