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. 2021 Sep 2;70(11):2506–2517. doi: 10.2337/db21-0181

Figure 4.

Figure 4

Hepatocytic ATF3 is a key regulator of lipolysis and fatty acid oxidation A–F: C57BL/6J mice were i.v. injected with AAV8-ALB-Null or AAV8-ALB-hATF3 and then fed the HFCF diet 16 weeks (n = 7 per group). A: Newly synthesized TG was determined after intraperitoneal injection of heavy water. Hepatic mRNA (B) and protein (C and D) levels were determined. Hepatic TGH activity (E) and plasma β-HB levels (F) were analyzed. G: FAO in hepatocytes was analyzed (n = 6). cpm, counts per min. H–L: Atf3fl/fl mice and Atf3Hep−/− mice were fed the HFCF diet for 16 weeks (n = 7). Hepatic mRNA (H) and protein (I and J) levels were determined. Hepatic TGH activity (K) and plasma β-HB levels (L) were analyzed. M: FAO in hepatocytes was determined (n = 6). All values are expressed as mean ± SEM. *P < 0.05, **P < 0.01 vs. controls.