Skip to main content
. 2021 Sep 8;8(21):2102418. doi: 10.1002/advs.202102418

Figure 2.

Figure 2

Mammary tumor organoid growth in alginate microbeads. a) Microfluidic chip channel under a 4× objective lens. b) Uniform‐sized fluorescent alginate microbeads. Scale bar: 400 µm. c) High‐throughput generation of mammary tumor organoids in alginate microbeads. Scale bar: 200 µm. d) Enlarged views of the luminal mammary tumor organoids in the microbeads. Scale bar: 100 µm. e) 3D schematic of a 140 µm luminal organoids: nuclear mapping within the Z‐axis (upper image) and cross‐sectional view at the z = 63 µm (bottom, right). Bright‐field image of the organoid (bottom, left). Scale bar: 10 µm. f) Growth of luminal organoids within five days. Scale bar: 100 µm. g) Percentages of alginate beads containing lumen only organoids, solid only organoids, lumen/solid organoids, and dispersed cells without organoids. h) Solid organoids with smooth boundary (I), blocky structures (II), “grape‐like” structures (III), and discohesive clusters (IV). Scale bar: 100 µm. i) Viability of the mammary tumor organoids in the alginate microbeads at day 14. Scale bar: 200 µm. j) Growth of bulk luminal organoids in the alginate microbeads (mean ± SD, n = 20, 24, 33, 40, respectively).