Figure 3.

Cell compartments of the organoids. a) Flow cytometry quantification of the cell lineages proportion within fresh PyMT tumors. Major lineages are defined by the expression of EpCAM (Epithelium), CD45 (leukocytes), and double‐negative (stroma). Luminal cells hierarchy is further defined by the expression of CD49f, CD49b, and Sca1. b) Flow cytometry results (as in panel (a)) for PyMT tumor organoids embedded in the alginate microbeads and cultured for two weeks. c) Expression of EpCAM, CD49f, and E‐cad in the luminal organoids. Scale bar: 50 µm, 10 µm (inset). d) Image of the structures with the lumen‐forming potential (I, II) and without the lumen‐forming potential (III). Scale bar: 20 µm.