Figure 5.

Organoid mechanical analysis in microbeads based on the TWPV theory. a) Luminal pressure and stretching stress of the luminal organoids in the alginate microbeads. b) Luminal expansion model based on TWPV theory and its main parameters. c) Bright‐field image of an organoid at the center of an alginate microbead and its expansion in fluorescent alginate microbeads. d) Fluorescence image of the organoids with monolayer cell connections. e) Lumen radius versus the luminal pressure (I) and tangential stress (II). f) Stress distribution on the microbeads when the luminal pressure is 2 kPa (①), and its distribution along the radius (II). g) Large organoids extruded from the alginate microbeads(②); luminal pressure leads to the hump exposed to the outside (II); large organoid released from the alginate microbeads (III). Scale bar: 100 µm.