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. 2021 Oct 20;12:730116. doi: 10.3389/fimmu.2021.730116

Figure 1.

Figure 1

Vaccine antigen gene analysis. (A) The workflow for selecting potential antigens combining proteomics with bioinformatics analysis. After “shaving” directly digested the surface protein, process the enzyme digested samples for LC-MS/MS to determine the cut-down proteins. Sequence conservation analysis predicted potential antigens. (B) Sequence conservation analysis. Comparing the genomic information of 389 Klebsiella pneumoniae strains obtained a conserved core gene database, which contains 562 proteins. The database was then analyzed from the structure information. The predicted surface proteins or secreted proteins were aligned with mass spectral data to exclude non-specific mass spectral data, resulting in the most potential antigens. (C) Conservative analysis of KOMPs. The amino acid sequence of these KOMPs (Kpn_Omp001, Kpn_Omp002, Kpn_Omp003, Kpn_Omp004, and Kpn_Omp005) identity ranges from 96% to 100%.