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Bacterial load assay. (A, B) Balb/c mice were immunized with the Kpn_Omp001, Kpn_Omp002, and Kpn_Omp005 individually or PBS, and they were injected with 5 × 108 CFU of K pneumoniae 260 in 7 days after the second booster vaccination. The lungs, kidneys, and spleens were collected 4 days after the sub-lethal challenge and homogenized. CFUs were enumerated following serial diluting and plating on BHI agar. *p < 0.05, **p < 0.01, ***p < 0.001.
