Figure 1.

scRNA-Seq description of immune-cell composition of the lactating mammary gland. (A) Schematic overview of the experimental pipeline used to perform the10X Chromium scRNA-Seq experiments on mammary tissue from lactating mice (n = 3). (B) Gating strategy used for FACS sorting prior to the use of single-cell Gel Beads in Emulsion (GEM). (C) UMAP plot of multiplexed CD45^pos cells isolated from the lactating mammary glands of two mice. The colors indicate clusters identified after automatic assignation. (D) Feature UMAP plots displaying the level of single-cell expression of key cell-type markers. (E) Heatmap indicating the five most differentially expressed genes for each CD45^pos cell cluster. (F) Proportion of CD45^pos immune cells in the lactating mammary gland determined by scRNA-seq. The numbers indicate the percentage of each cell type among all immune CD45^pos cells.