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. 2021 Oct 20;12:754661. doi: 10.3389/fimmu.2021.754661

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Copyright © 2021 Hassel, Gausserès, Guzylack-Piriou and Foucras

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immune-cell composition of the lactating mammary gland. (A, C) Flow cytometry analysis of the immune landscape on single-cell suspension from lactating mammary tissue. The numbers indicate the percentage of cells within the corresponding gate. These patterns are representative of one of 10 individual mice analyzed, each giving similar results. (B) Pie chart showing the proportions of CD45^pos immune cells in the lactating mammary gland by flow cytometry. The numbers indicate the percentage of each cell type among all living CD45^pos immune cells. (C) Flow cytometry analysis of the level of various markers in F4/80 CD11c double-negative, single-positive, and double-positive CD45^pos cell sub-populations.