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. 2021 Oct 20;11:756779. doi: 10.3389/fonc.2021.756779

Figure 2.

Figure 2

AR may decrease HCC progression via altering miR-122-5p expression. (A) 9 downregulated miRNAs was selected from the published papers. (B, C) qRT-PCR assay was used to check the miRNAs expression after shAR/oeAR in HCC cells. (D) Transwell invasion assay was used to check the invasion capacity of HCC Hep3B cells transfected with pWPI/oeAR after adding miR-122-5p inhibitor or miR-192-5p inhibitor in the cells. (E) Wound healing migration assay was used to check HCC Hep3B cells migration capacity after oeAR/adding miR-122-5p inhibitor in the cells. (F, G) MTT and colony formation assays were used to check HCC Hep3B cells proliferation capacities after oeAR/adding miR-122-5p inhibitor in the cells. (H) Transwell invasion assay was used to check HCC PLC/PRF/5 cells invasion capacity after shAR/oemiR-122-5p in the cells.