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. 2021 Oct 20;11:755433. doi: 10.3389/fonc.2021.755433

Figure 1.

Figure 1

Effect of Cabozantinib on proliferation of DU-145 and PC-3 prostate tumor cells. (A) MTT proliferation assay of cells treated for 24 or 48 h with 2.5 and 5 μg/ml of Cabozantinib. Histograms represent the mean values of optical density (OD) 550 nm of three independent experiments (triplicate each condition) ± SEM. (B) Apoptosis of cells treated for 24 or 48 h with Cabozantinib (2.5 and 5 μg/ml). Histograms represent the mean values of percentage of apoptotic cells identified as Annexin positive of three independent experiments ± SEM. (C) Cell cycle analysis by propidium iodide (PI) staining analyzed by flow cytometry. One representative analysis out of three experiments is shown. The histograms represent the count of PI-positive cells found in untreated (NT) and Cabozantinib-treated (2.5 and 5 μg/ml) DU-145 and PC-3 cells as analyzed by FACS DIVA software. Gates allow the identification of phase G1, S, and G2 cell cycle. NT, untreated cells. *p < 0.05; **p < 0.01, Student’s t-test.