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. 2021 Oct 20;11:755433. doi: 10.3389/fonc.2021.755433

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Copyright © 2021 Scirocchi, Napoletano, Pace, Rahimi Koshkaki, Di Filippo, Zizzari, Nuti and Rughetti

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Flow cytometry analysis to evaluate Calreticulin (CRT) cell membrane expression in DU-145 and PC-3 prostate cancer cells treated with Cabozantinib (2.5 and 5 μg/ml) or 24 and 48 h. Dot plots show the analysis of cell population considering the 7-ADD vs. CRT staining (y- vs. x-axis, respectively). Histograms represent the ratio (T/NT) between the average of percentage of CRT⁺ cells in Cabozantinib-treated cells (2.5 and 5 μg/ml) vs. untreated cells ± SEM, evaluated in three independent experiments. *p < 0.05; **p < 0.01; ****p < 0.0001, Student’s t-test. NT, untreated cells as experimental control. ns, not significant.