Figure 2.

(A) The CLSM images of 4T1 cells under different treatment in normoxia condition. The nuclei were stained with DAPI (blue) and DSBs were stained with γ-H₂AX (red). (B) The CLSM images of 4T1 cells under different treatment in hypoxia condition. The nuclei were stained with DAPI (blue) and DSBs were stained with γ-H₂AX (red). (C) The density of γ-H₂AX foci in (A) was determined based on analyses of 100 cells per treatment group (γ-H₂AX foci/100 μm², n = 3). (D) The density of γ-H₂AX foci in (B) was determined based on analyses of 100 cells per treatment group (γ-H₂AX foci/100 μm², n = 3). (E) Colony formation assays were conducted using 4T1 cells treated with radiation under normoxia condition (n = 3). (F) Colony formation assays were conducted using 4T1 cells treated with radiation under hypoxia condition (n = 3). (G) Dark cytotoxicity of SFO on 4T1 cells. (H) Quantitative analysis of GSH levels for different SFO concentration. **P < 0.01; Student’s t-test.