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. 2021 Oct 20;12:769799. doi: 10.3389/fimmu.2021.769799

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Copyright © 2021 Tagliamonte, Mauriello, Cavalluzzo, Ragone, Manolio, Luciano, Barbieri, Palma, Scognamiglio, Di Mauro, Di Bonito, Tornesello, Buonaguro, Vitagliano, Caporale, Ruvo and Buonaguro

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Number of CD3⁺ Granzyme B⁺ and FoxP3⁺ cells infiltrating B16F10 tumors. Cells are represented as mean with standard deviation (SD) in each experimental group. (A) CD3⁺, Granzyme B⁺ and FoxP3⁺cells in CT (A) and IM (B) area by IHC are shown, respectively. (C) Cumulative evaluation of the effector cytotoxic GrzB+ and suppressive FOXP3+ T cell on total CD3+ cells. Average standard deviation of the percentage of necrosis in each experimental group is shown (D). Statistical analysis 2way ANOVA: *p < 0.05, **p < 0.005, ***p < 0,001, ****p < 0,0001.