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. 2021 Feb 19;42(11):1875–1887. doi: 10.1038/s41401-021-00612-9

Fig. 2. The combination of lovastatin and 2DG triggers significantly increased apoptosis rates.

Fig. 2

The combination of lovastatin and 2DG triggers significantly increased apoptosis rates. After treatment with lovastatin (15 μM) and 2DG (5 mM) alone or in combination for 48 h, a HCT116 and LoVo cells were stained with FITC Annexin V/PI, and apoptosis was quantified by flow cytometry. b Quantification data of the apoptotic cells. c Representative immunoblots of cleaved caspase-3 and cleaved PARP in the HCT116 and LoVo cells. β-actin served as a loading control. d Quantified data of (c). e HCT116 and LoVo cells were treated with lovastatin (15 μM) and 2DG (5 mM) alone or in combination for 48 h. Representative immunoblots of AKT, p-AKT, p-ERK, and ERK. β-actin served as a loading control. f Quantified data of (e). All data are from at least three independent experiments. *P < 0.05, **P < 0.01.