Fig. 3. The combination of lovastatin and 2DG induces G₂/M cell cycle arrest.

HCT116 and LoVo cells were treated with lovastatin (15 μM) and 2DG (5 mM) alone or in combination for 24 h. a Cell cycle distribution was analyzed using flow cytometry. b Percentage of cells in the G₁, S, and G₂/M phase. c Representative immunoblots showing the indicated proteins. β-actin served as a loading control. d Quantified data of (c). All data are from at least three independent experiments. *P < 0.05, **P < 0.01.