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. 2021 May 15;28(11):2991–3008. doi: 10.1038/s41418-021-00796-x

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© The Author(s), under exclusive licence to ADMC Associazione Differenziamento e Morte Cellulare 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — An MA plot shows the reproducibility of 13,924 reactions between two repeats in the ALPHA. Each point represents the log₂ fold change (0.5–2) in two repeats. The red dashed lines represent the fold change threshold (±1). A total of 98.74% of the points were located between the two red dashed lines, demonstrating that this assay was highly reproducible and reliable.