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. 2021 May 15;28(11):2991–3008. doi: 10.1038/s41418-021-00796-x

Fig. 7. An immunoprecipitation assay confirms the ALPHA results.

Fig. 7

A ALPHA results of the four domain–domain interaction pairs examined in panels B and C. B, C An AGIA-tagged domain and a FLAG-tagged domain were co-expressed in HEK293T cells. Soluble supernatants generated from whole cell lysates (input) were applied to immunoprecipitation assay using an anti-AGIA or an anti-FLAG antibody, and co-immunoprecipitants were detected by immunoblotting with an anti-FLAG or anti-AGIA antibody, respectively.